human glioma cell lines u251  (Procell Inc)

Journal: Materials Today Bio

Article Title: Doxorubicin-loaded PEGylated liposome modified with ANGPT2-specific peptide for integrative glioma-targeted imaging and therapy

doi: 10.1016/j.mtbio.2025.101455

Figure Lengend Snippet: High ANGPT2 expression correlates with GBM progression and poorer prognosis. (A) ANGPT2 mRNA expression in different malignant tumors ( http://gepia.cancer-pku.cn/ ). (B) Comparison of ANGPT2 mRNA expression between normal brain and GBM tissues from TCGA datasets. (C) ANGPT2 mRNA expression in brain tumor at different stages ( http://www.cgga.org.cn/ ), # p < 0.001. (D) ANGPT2 mRNA expression in brain tumor at WHO stage IV in individuals of different gender and age ( http://www.cgga.org.cn/ ). (E) Kaplan-Meier curves estimating overall survival and (F) disease-free survival ( https://smuonco.shinyapps.io/PanCanSurvPlot ) in patients of ANGPT2 mRNA in GBM. Log-rank test, p < 0.001. (G) The observation of ANGPT2 protein expression in different stages of brain tumor tissues. (H) Representative images of ANGPT2 protein expression (a, c, e, and g, magnification 5 × ; b, d, f, and h, magnification 200 × , bar = 50 μm). (I) Positive rate of ANGPT2 in glioma in different stages of brain tumor tissues. (J, K) ANGPT2 expression was analyzed using IB in HA, T98G, U251, and U87-MG cells. ANGPT2 expression was quantified (ANGPT2/β-actin). Normalized ANGPT2 in HA cells was set at 1.0. ∗ p < 0.05, ∗∗ p < 0.01 ( n = 3). (L, M) ANGPT2 levels in membrane proteins were analyzed using IB in T98G, U251, and U87-MG cells. The level of ANGPT2 was quantified (ANGPT2/Na + -K + ATPase). IB, immunoblotting; HA, human astrocyte cell.

Article Snippet: U251 (The human malignant glioma cell lines, no. CL-0237) (Procell, China), HA (The human astrocyte cell lines, no.1800) (ScienCell Research Laboratories, San Diego, CA, USA), U87-MG (The human malignant glioma cell lines, no. CL-0238) (Procell, China), U87-MG-luc (The human astrocyte tumors cell line, no. WZ0028) (Fenghui Bio, Changsha, China) and bEND.3 (The mouse brain-derived endothelial cells.3, no. TCM-C715) (Hycyte Biotechnology, Suzhou, China) were cultured in DMEM medium with 10 % fetal bovine serum.

Techniques: Expressing, Comparison, Membrane, Western Blot

Journal: Frontiers in Molecular Neuroscience

Article Title: High expression of COPZ2 is associated with poor prognosis and cancer progression in glioma

doi: 10.3389/fnmol.2024.1438135

Figure Lengend Snippet: Knockdown of COPZ2 inhibited the malignant phenotypes of glioma cells. The knockdown efficiency of COPZ2 was detected in (A) U87 and (B) U251 cells. (C) The knockdown efficiency of COPZ2 in U87 and U251 glioma cells were detected by western blot. The proliferation capacity of (D) U87 and (E) U251 cells after COPZ2 knockdown at 24 h, 48 h, and 72 h. (F) The wound healing assay of the effect of COPZ2 knockdown on the U87 and U251 cell migration. (G) The transwell assay of the effect of COPZ2 knockdown on the U87 and U251 cell invasion. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Human glioma cell lines U251 and U87 cell lines were obtained from the China Center for Type Culture Collection (CCTCC, China).

Techniques: Knockdown, Western Blot, Wound Healing Assay, Migration, Transwell Assay

Journal: Frontiers in Molecular Neuroscience

Article Title: High expression of COPZ2 is associated with poor prognosis and cancer progression in glioma

doi: 10.3389/fnmol.2024.1438135

Figure Lengend Snippet: AKT activation reversed the antioncogenic effect of COPZ2 knockdown. The CCK-8 assay (A,B) , wound healing assay (C) , and transwell assay (D) of U87 and U251 cells after transfected with si-COPZ2 in the presence or absence of SC79 (5 μg/mL). * p < 0.05, ** p < 0.01, *** p < 0.001 vs. NC group; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. si-COPZ2 group.

Article Snippet: Human glioma cell lines U251 and U87 cell lines were obtained from the China Center for Type Culture Collection (CCTCC, China).

Techniques: Activation Assay, Knockdown, CCK-8 Assay, Wound Healing Assay, Transwell Assay, Transfection